We have organized this Special Issue to discuss the appropriate possibilities for skin reconstruction and regeneration. Luze et al. explained how antiseptic-loaded BNC represents a promising and effective approach for the treatment of biofilms. Korzeniowski et al. demonstrated how a bromelain-based enzymatic method offers a good, safe debridement option to improve the life quality of children with severe burns. -Martinovic et al. wrote a paper titled “Objective Skin Quality Assessment after Reconstructive Procedures for Facial Skin Defects”. An interesting technique was introduced by Woo Yoo et al.—a modified mini-keystone flap technique for covering tiny volar pulp defects of the fingertip, with few complications and favorable outcomes. Russo et al. conducted a comparative study between Connettivina Bio Plus and Fitostimoline Plus. -They showed that both were secure and effective for treating acute superficial skin lesions. Hwan Kim et al. published “A Prospective Randomized Trial Comparing Paste-Type Acellular Dermal Matrix to Standard Care for the Treatment of Chronic Wounds”. Korzeniowski et al. enriched the Special Issue with a systematic review titled “The Role of the Immune System in Pediatric Burns”.-Finally we presented the paper “Collection of Key Surgical Techniques for the Reconstruction of Midface Skin Defects” for the midface’s significant role in defining important facial traits. Finally, I would like to express my sincere respect to all authors for submitting excellent papers, and to reviewers for taking the time to review them.